A structural view of synthetic cofactor integration into [FeFe]-hydrogenases

Computational studies of [NiFe] and [FeFe] hydrogenases.

A synthetic system links FeFe-hydrogenases to essential E. coli sulfur metabolism

BACKGROUND FeFe-hydrogenases are the most active class of H2-producing enzymes known in nature and may have important applications in clean H2 energy production. Many potential uses are currently complicated by a crucial weakness: the active sites of all known FeFe-hydrogenases are irreversibly inactivated by O2. RESULTS We have developed a synthetic metabolic pathway in E. coli that links Fe...

A Cell-Free Microtiter Plate Screen for Improved [FeFe] Hydrogenases

BACKGROUND [FeFe] hydrogenase enzymes catalyze the production and dissociation of H(2), a potential renewable fuel. Attempts to exploit these catalysts in engineered systems have been hindered by the biotechnologically inconvenient properties of the natural enzymes, including their extreme oxygen sensitivity. Directed evolution has been used to improve the characteristics of a range of natural ...

High-Yield Expression of Heterologous [FeFe] Hydrogenases in Escherichia coli

BACKGROUND The realization of hydrogenase-based technologies for renewable H(2) production is presently limited by the need for scalable and high-yielding methods to supply active hydrogenases and their required maturases. PRINCIPAL FINDINGS In this report, we describe an improved Escherichia coli-based expression system capable of producing 8-30 mg of purified, active [FeFe] hydrogenase per ...

a structural survey of the polish posters

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